Core Facilities
Flow cytometry

The Flow Cytometry Core Facility at the Institute for Experimental and Clinical Cell Therapy offers both conventional and spectral flow cytometry, enabling multiparametric analysis of cells in terms of phenotype, cell cycle status, proliferation, or viability.

For conventional flow cytometry, the facility provides access to a Beckman Coulter Gallios and a Becton Dickinson LSR Fortessa. The Gallios is equipped with three lasers and allows for the simultaneous measurement of up to 10 fluorescent parameters. It also features a sample carousel that can hold up to 32 samples for automated measurements. The LSR Fortessa, with its five lasers, enables the simultaneous analysis of up to 18 fluorescent parameters. For highly complex phenotypes, identifying small subpopulations, or analyzing highly autofluorescent samples, the facility offers a state-of-the-art Cytek Northern Lights system for spectral flow cytometry.

Additionally, cell sorting is available for tasks such as enriching specific cell populations or separating successfully transfected cells. For this, a Becton Dickinson FACS Aria III with 4 lasers and a capacity for up to 13 fluorescent parameters is available.

We provide users with consultation and support in panel design, experiment planning, and data analysis.

Terms of Use

The Flow Cytometry Core Facility is open to both internal and external partners. For independent use, mandatory training on the respective instrument and approval from the designated contact person are required. Users are responsible for providing their own consumables.

For more information, please contact Dr. Maeding.